Custom Antibody Engineering
AbYBiotech specialises in the development and production of custom-designed polyclonal, monoclonal and recombinant antibodies. Polyclonal antibodies are produced using a variety of different hosts including rabbits, chickens and mice.
A particular strength is the development of recombinant antibodies, so-called antibody fragments, which can be engineered to have higher affinity and higher specificity than their monoclonal or polyclonal counterparts. AbYBiotech has the option to use either phage display or its own proprietary technology in the development of recombinant antibodies.
AbYBiotech has successfully produced antibodies for the following target applications:
Recombinant Antibody Engineering
Whilst AbYBiotech produces polyclonal, monoclonal and recombinant antibodies (rAb), it has particular expertise in rAb development. Recombinant antibodies can be used in all applications in which traditional mAb are used and have inherent advantages over them.
In our lab we use phage display in combination with immune cDNA libraries to successfully produce large and diverse panels of recombinant antibodies. The power of phage display lies in the precise control over selection conditions which can be tailored to retrieve antibody fragments that have high-affinity and specificity. In addition, the genetic coding material of the antibody fragment resides within the phage particle and therefore, during selection a direct link between the antibody’s genotype and phenotype is maintained. Thus, the genes encoding the antibody fragment can be readily retrieved from the phage DNA. We have successfully applied phage display to a wide variety of target molecules. In addition, through the use of antibody engineering techniques we have successfully generated antibodies with enhanced sensitivity and specificity.
Uses of Recombinant Antibodies
As with traditional animal-generated antibodies, rAbs can be used in blotting techniques (Western, Northern, Southern, etc.), immunostaining, flow cytometry, and enzyme linked immunosorbent assays (ELISAs). rAbs are also used in the diagnosis of infectious diseases by detecting pathogens and can be used in protein arrays that analyze relative protein expression associated with disease or other biological change. Regulatory agencies have also realized the utility of rAbs in vaccine potency testing and in therapeutics.