Anti-free Prostate Specific Antigen (fPSA) scFv (B8H8)

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Overview

Antigen Target

Prostate Specific Antigen (fPSA)

Epitope

See reference 1

Antibody type/clonality

Monoclonal scFv

Clone Number

B8H8

Source/Host

Chicken

Species Reactivity

Human

Applications

ELISA, WB

Properties

Form

Liquid

Purity

Specificity

Affinity

Concentration

1.6 mg/mL

Application working concentration

ELISA (1:1,000 – 1:10,000)

Storage Buffer

1x PBS

Supply & Storage

Storage Conditions

-20oC in 1 x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

100 μL

Shipping Conditions

Additional Information

Reference on Antibody

1. Conroy, P. J., Law, R. H., Gilgunn, S., Hearty, S., Caradoc-Davies, T. T., Lloyd, G., ... & Whisstock, J. C. (2014). Reconciling the Structural Attributes of Avian Antibodies. Journal of Biological Chemistry, 289(22), 15384-15392.

2. Spain, E., Gilgunn, S., Sharma, S., Adamson, K., Carthy, E., O’Kennedy, R., & Forster, R. J. (2016). Detection of prostate specific antigen based on electrocatalytic platinum nanoparticles conjugated to a recombinant scFv antibody. Biosensors and Bioelectronics, 77, 759-766.

Application Information

SDS-PAGE and Western blotting analysis of anti-fPSA scFv B8H8

Fig. 1 SDS-PAGE and Western Blot Analysis of anti-fPSA.

Figure 1A shows an SDS-PAGE coomassie stained gel containing the scFv (~ 25 kDa). The figure displays first the page ruler (PR), Concentrated Protein and a 1:10 dilution of the antibody. Figure 1B shows a Western blot image probe with anti- HA-HRP confirming the presence of the scFv (~ 25 kDa). The figure displays first the page ruler (PR), Concentrated Protein and a 1:10 dilution of the antibody.

Contact Information

Name

Dr Jenny Fitzgerald

Telephone

+353 1 700 7556

E-mail

jenny.fitzgerald@dcu.ie

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

Download