Anti-heart-type Fatty Acid Binding Protein (hFABP) scFv (3G9)

PDF Spec Sheet

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Overview

Antigen Target

Human heart-type fatty acid binding protein (hFABP)

Epitope

Not defined

Antibody type/clonality

Recombinant scFv antibody

Clone Number

3G9

Source/Host

Chicken

Species Reactivity

Applications

ELISA, WB

Properties

Form

Liquid

Purity

Specificity

High

Affinity

8.36 X 10-10M

Concentration

1.32 mg/mL

Application working concentration

ELISA (1:1,000-1:10,000)
Western blotting (1:1,000-1:10,000)

Storage Buffer

1 x PBS plus 5% Glycerol

Supply & Storage

Storage Conditions

-20oC in 1 x PBS plus 5% Glycerol. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

100 μL

Shipping Conditions

Additional Information

Reference on Antibody

Ayyar B.V., Hearty S. and O'Kennedy R. (2010) Highly sensitive recombinant antibodies capable of reliably differentiating heart-type fatty acid binding protein from noncardiac isoforms. Anal. Biochem., 407(2):165-71.

Application Information

SDS-PAGE and Western blotting analysis of anti-hFABP, 3G9 (Fig.1-2)

Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-hFABP.

Figure 1a shows the SDS-PAGE containing purified anti-hFABP scFv (~ 28 kDa) and the anti-hFABP scFv-specific band on Western blotting was probed with mouse anti-HA IgG. MW: Pre-stained protein ladder; E: elution. The figure 1b shows the schematic diagram of the Western blotting analysis.

Fig. 2 Detection of hFABP using chicken anti-hFABP, 3G9.

Figure 2a shows the SDS-PAGE containing purified hFABP (~ 15 kDa) and the hFABP-specific band on Western blotting was probed with chicken anti-hFABP scFv, 3G9 followed by HRP-labelled anti-HA IgG. MW: Pre-stained protein ladder; F-T: ‘flow through’; W: wash; E: elution. The figure 2b shows the schematic diagram of the Western blotting analysis.

Contact Information

Name

Dr Caroline Murphy

Telephone

+353 1 700 8324

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

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