Application working concentration
Western blotting (1:1,000-1:10,000)
Supply & Storage
Standard Shipping Volumes
Reference on Antibody
SDS-PAGE and Western blotting analysis of anti-hFABP, 3G9 (Fig.1-2)
Figure 1a shows the SDS-PAGE containing purified anti-hFABP scFv (~ 28 kDa) and the anti-hFABP scFv-specific band on Western blotting was probed with mouse anti-HA IgG. MW: Pre-stained protein ladder; E: elution. The figure 1b shows the schematic diagram of the Western blotting analysis.
Figure 2a shows the SDS-PAGE containing purified hFABP (~ 15 kDa) and the hFABP-specific band on Western blotting was probed with chicken anti-hFABP scFv, 3G9 followed by HRP-labelled anti-HA IgG. MW: Pre-stained protein ladder; F-T: ‘flow through’; W: wash; E: elution. The figure 2b shows the schematic diagram of the Western blotting analysis.
Dr Caroline Murphy
+353 1 700 8324
School of Biotechnology,
Dublin City University,