Anti-Human cardiac troponin I (cTnI) Fab (4G5)

PDF Spec Sheet

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Overview

Antigen Target

Human cardiac troponin I (cTnI)

Epitope

QPLELAGLGFAELQ

Antibody type/clonality

Recombinant Fab antibody

Clone Number

4G5

Source/Host

Chicken

Species Reactivity

Applications

ELISA, WB, Biacore

Properties

Form

Liquid

Purity

Specificity

Affinity

1.55 ± 0.82 X 10-9M

Concentration

0.28 mg/mL

Application working concentration

ELISA (1:100-1:500)
Western blotting (1:100-1:500)

Storage Buffer

1x PBS

Supply & Storage

Storage Conditions

-20oC in 1x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

200 μL

Shipping Conditions

Additional Information

Reference on Antibody

Conroy, P.J. (2011) Exploiting novel antibodies for the early detection of cardiac disease. PhD thesis, Dublin City University.

Application Information

SDS-PAGE, Western blotting and Biacore analysis of anti-cTnI Fab, 4G5 (Fig.1-2)

Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-cTnI Fab.

Figure 1a shows the SDS-PAGE containing purified anti-cTnI Fab (~ 50 kDa) and the anti-cTnI Fab-specific band on Western blotting was probed with mouse anti-HA IgG. PR: Pre-stained protein ladder.

Fig. 2 shows the specific band on Western blotting (~23kDa) of the cTnI antigen probed with the anti- cTnI FAB. PR: Pre-stained Page Ruler; cTnI: cTnI Antigen. Figure 2b shows the schematic diagram of Western blotting analysis.

Fig. 3 Kinetic analysis of interactions between anti-cTnI Fab and cTnI using the Biacore 3000 system.

The coloured lines represent the response data of various concentrations of cTnI. The black lines represent the localised fit to the response data, shown as coloured lines.

Contact Information

Name

Dr Hui Ma

Telephone

+353 1 700 8325

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

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