SDS-PAGE, Western blotting and Biacore analysis of anti-cTnI Fab, 4G5 (Fig.1-2)
Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-cTnI Fab.
Figure 1a shows the SDS-PAGE containing purified anti-cTnI Fab (~ 50 kDa) and the anti-cTnI Fab-specific band on Western blotting was probed with mouse anti-HA IgG. PR: Pre-stained protein ladder.
Fig. 2 shows the specific band on Western blotting (~23kDa) of the cTnI antigen probed with the anti- cTnI FAB. PR: Pre-stained Page Ruler; cTnI: cTnI Antigen. Figure 2b shows the schematic diagram of Western blotting analysis.
Fig. 3 Kinetic analysis of interactions between anti-cTnI Fab and cTnI using the Biacore 3000 system.
The coloured lines represent the response data of various concentrations of cTnI. The black lines represent the localised fit to the response data, shown as coloured lines.