Anti-Human cardiac troponin I (cTnI) scAb (4G5)

PDF Spec Sheet

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Overview

Antigen Target

Human cardiac troponin I (cTnI)

Epitope

QPLELAGLGFAELQ

Antibody type/clonality

Recombinant scAb antibody

Clone Number

4G5

Source/Host

Chicken

Species Reactivity

Applications

ELISA, WB, Biacore

Properties

Form

Liquid

Purity

Specificity

Affinity

2.95 ± 1.25 × 10-9M

Concentration

0.8 mg/mL

Application working concentration

ELISA (1:1,000-1:10,000)
Western blotting (1:1,000- 1:10,000)

Storage Buffer

1 x PBS

Supply & Storage

Storage Conditions

-20oC in 1 x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

200 μL

Shipping Conditions

Additional Information

Reference on Antibody

Conroy, P.J. (2011) Exploiting novel antibodies for the early detection of cardiac disease. PhD thesis, Dublin City University.

Application Information

SDS-PAGE, Western blotting and biacore analysis of anti-cTnI scAb, 4G5 (Fig.1-2)

Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-cTnI scAb.

Figure 1a shows the SDS-PAGE containing purified anti-cTnI scAb (~ 40 kDa). The Western blot shows the specific band of the cTnI antigen (~23kDa) probed with the anti-cTnI scFv. PR: Pre-stained Page Ruler; cTnI: cTnI Antigen. Figure 1b shows the schematic diagram of Western blotting analysis.

Fig. 2 Kinetic analysis of interactions between anti-cTnI scAb and cTnI using the Biacore 3000 system.

The coloured lines represent the response data of various quantities of cTnI. The black lines represent the localised fit to the response data, shown as coloured lines.

Contact Information

Name

Dr Hui Ma

Telephone

+353 1 700 8325

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

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