SDS-PAGE, Western blotting and Biacore analysis of anti-cTnI scFv, 4G5 (Fig.1-2)
Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-cTnI scFv 4G5.
Figure 1a shows the SDS-PAGE containing purified anti-cTnI scFv (~28 kDa) and the anti-cTnI scFv-specific band on Western blotting which was probed with mouse anti-HA IgG. PR: Pre-stained Page Ruler; scFv: eluted scFv. Figure 1b shows the schematic diagram of Western blotting analysis.
Fig. 2 Western blotting analysis of cTnI antigen probed with the anti-cTnI scFv 4G5.
Figure 2a shows the specific band on Western blotting (~23kDa) of the cTnI antigen probed with the anti-cTnI scFv. PR: Pre-stained Page Ruler; cTnI: cTnI Antigen. Figure 2b shows the schematic diagram of Western blotting analysis.
Fig. 3 Kinetic analysis of interactions between anti-cTnI scFv 4G5 and cTnI using the Biacore 3000 system.
The coloured lines represent the response data related to various concentrations of cTnI. The black lines represents the localised fit to the response data, shown as coloured lines.