Anti-Human cardiac troponin I (cTnI) scFv (4G5)

PDF Spec Sheet

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Overview

Antigen Target

Human cardiac troponin I (cTnI)

Epitope

Antibody type/clonality

Recombinant scFv antibody

Clone Number

4G5

Source/Host

Chicken

Species Reactivity

Applications

ELISA, WB, Biacore

Properties

Form

Liquid

Purity

Specificity

Affinity

1.16 ± 0.69 x 10-9M

Concentration

0.18 mg/mL

Application working concentration

ELISA (1:10,000-1:100,000)
Western blotting (1:5,000-1:50,000)

Storage Buffer

1 x PBS

Supply & Storage

Storage Conditions

-20oC in 1 x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

200 μL

Shipping Conditions

Additional Information

Reference on Antibody

Conroy, P.J. (2011) Exploiting novel antibodies for the early detection of cardiac disease. PhD thesis, Dublin City University.

Application Information

SDS-PAGE, Western blotting and Biacore analysis of anti-cTnI scFv, 4G5 (Fig.1-2)

Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-cTnI scFv 4G5.

Figure 1a shows the SDS-PAGE containing purified anti-cTnI scFv (~28 kDa) and the anti-cTnI scFv-specific band on Western blotting which was probed with mouse anti-HA IgG. PR: Pre-stained Page Ruler; scFv: eluted scFv. Figure 1b shows the schematic diagram of Western blotting analysis.

Fig. 2 Western blotting analysis of cTnI antigen probed with the anti-cTnI scFv 4G5.

Figure 2a shows the specific band on Western blotting (~23kDa) of the cTnI antigen probed with the anti-cTnI scFv. PR: Pre-stained Page Ruler; cTnI: cTnI Antigen. Figure 2b shows the schematic diagram of Western blotting analysis.

Fig. 3 Kinetic analysis of interactions between anti-cTnI scFv 4G5 and cTnI using the Biacore 3000 system.

The coloured lines represent the response data related to various concentrations of cTnI. The black lines represents the localised fit to the response data, shown as coloured lines.

Contact Information

Name

Dr Hui Ma

Telephone

+353 1 700 8325

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

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