Application working concentration
Western blotting (1:5,000-1:50,000)
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Reference on Antibody
SDS-PAGE, Western blotting and Biacore analysis of anti-cTnI scFv, 4G5 (Fig.1-2)
Figure 1a shows the SDS-PAGE containing purified anti-cTnI scFv (~28 kDa) and the anti-cTnI scFv-specific band on Western blotting which was probed with mouse anti-HA IgG. PR: Pre-stained Page Ruler; scFv: eluted scFv. Figure 1b shows the schematic diagram of Western blotting analysis.
Figure 2a shows the specific band on Western blotting (~23kDa) of the cTnI antigen probed with the anti-cTnI scFv. PR: Pre-stained Page Ruler; cTnI: cTnI Antigen. Figure 2b shows the schematic diagram of Western blotting analysis.
The coloured lines represent the response data related to various concentrations of cTnI. The black lines represents the localised fit to the response data, shown as coloured lines.