Anti-Internalin A (InlA) mAb (2B3)

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Overview

Antigen Target

Internalin A (InlA)

Epitope

Antibody type/clonality

Monoclonal IgG antibody

Clone Number

2B3

Source/Host

Mouse

Species Reactivity

Applications

ELISA, WB

Properties

Form

Liquid

Purity

Specificity

Affinity

Concentration

1.93 mg/mL

Application working concentration

ELISA (1:1,000-1:10,000)
Western blotting (1:500- 1:5,000)

Storage Buffer

1 x PBS

Supply & Storage

Storage Conditions

-20oC in 1 x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

200 μL

Shipping Conditions

Additional Information

Reference on Antibody

Hearty S., Leonard P., Quinn J., O'Kennedy R. ‘Production, characterisation and potential application of a novel monoclonal antibody for rapid identification of virulent Listeria monocytogenes’. Journal of Microbiological Methods. 2006;66(2):294- 312

Tully, E., Hearty, S., Leonard, P. and O’Kennedy, R., 2006. The development of rapid fluorescence-based immunoassays, using quantum dot-labelled antibodies for the detection of Listeria monocytogenes cell surface proteins. International journal of biological macromolecules, 39(1), 127-134.

Gilmartin, N., Gião, M.S., Keevil, C.W. and O'Kennedy, R., 2016. Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions. International journal of food microbiology, 219, 50-55.

Application Information

SDS-PAGE and Western blotting analysis of anti-InlA mAb, 2B3 (Fig.1-2).

Fig. 1 SDS-PAGE analysis of anti-InlA mAb, 2B3.

The figure shows the SDS-PAGE containing the light (~ 25 kDa) and heavy chains (~ 50 kDa) of anti-InlA mAb, 2B3.

Fig. 2 Detection of InlA using mouse anti-InlA mAb, 2B3.

Figure 2a shows the SDS-PAGE containing purified InlA (~ 80 kDa) and the InlA-specific band on Western blotting was probed with mouse anti- InlA mAb, 2B3 followed by HRP-labelled anti-mouse IgG. MW: Pre-stained protein ladder; CP: Concentrated Protein. The figure 2b shows the schematic diagram of the Western blotting analysis.

Contact Information

Name

Dr Jenny Fitzgerald

Telephone

+353 1 700 7556

E-mail

jenny.fitzgerald@dcu.ie

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

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