Application working concentration
Western blotting (1:500- 1:5,000)
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Reference on Antibody
Tully, E., Hearty, S., Leonard, P. and O’Kennedy, R., 2006. The development of rapid fluorescence-based immunoassays, using quantum dot-labelled antibodies for the detection of Listeria monocytogenes cell surface proteins. International journal of biological macromolecules, 39(1), 127-134.
Gilmartin, N., Gião, M.S., Keevil, C.W. and O'Kennedy, R., 2016. Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions. International journal of food microbiology, 219, 50-55.
SDS-PAGE and Western blotting analysis of anti-InlA mAb, 2B3 (Fig.1-2).
The figure shows the SDS-PAGE containing the light (~ 25 kDa) and heavy chains (~ 50 kDa) of anti-InlA mAb, 2B3.
Figure 2a shows the SDS-PAGE containing purified InlA (~ 80 kDa) and the InlA-specific band on Western blotting was probed with mouse anti- InlA mAb, 2B3 followed by HRP-labelled anti-mouse IgG. MW: Pre-stained protein ladder; CP: Concentrated Protein. The figure 2b shows the schematic diagram of the Western blotting analysis.
Dr Jenny Fitzgerald
+353 1 700 7556
School of Biotechnology,
Dublin City University,