Anti-Microcystin-LR (MC-LR) scFv (R66S)

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Overview

Antigen Target

Microcystin-LR (MC-LR)

Epitope

Antibody type/clonality

Recombinant antibody fragment (single chain fragment variable (scFv))

Clone Number

R66S

Source/Host

Mouse

Species Reactivity

Applications

ELISA, WB, Mbio lateral flow fluorescence-based immunoassay

Properties

Form

Liquid

Purity

Specificity

Binds MC-LR 100%, MC-YR 79.7%, MC-LR 74.8%, NOD 69.3%, MC-LF 67.5%, MC-LW 63.7%, MC-RR 60.1%.

Affinity

Concentration

2.78 mg/mL

Application working concentration

ELISA (1:1,000-1:10,000)
Western blotting (1:500- 1:5,000)
Mbio lateral flow immunoassay (1:10,000)

Storage Buffer

1 x PBS

Supply & Storage

Storage Conditions

-20oC in 1 x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

200 μL

Shipping Conditions

Additional Information

Reference on Antibody

Murphy C., Stack E., Krivelo S., McPartlin D.A., Byrne B., Greef C., Lochhead MJ., Husar G., Devlin S., Elliott C.T., O'Kennedy R.J. ‘Detection of the cyanobacterial toxin, microcystin-LR, using a novel recombinant antibody-based optical-planar waveguide platform.’ Biosensors and Bioelectronics. 2014; 15(67):708-14.

Application Information

SDS-PAGE and fluorescence immunoassay and analysis of anti-MC-LR scFv (Fig.1-2).

Fig. 1 SDS-PAGE and Western Blot Analysis of anti-MC-LR.

Figure 1A shows an SDS-PAGE coomassie stained gel containing the scFv (~ 25 kDa). The figure displays first the page ruler (PR), Concentrated Protein and a 1:10 dilution of the antibody. Figure 1B shows a Western blot image probe with anti-HA-HRP confirming the presence of the scFv (~ 25 kDa). The figure displays first the page ruler (PR), Concentrated Protein and a 1:10 dilution of the antibody.

Fig. 2 A fluorescence image of anti-MC-LR scFv-Alexa647 bound to MC-LR-BSA conjugate 50 μg/mL and 10 μg/mL, the image was taken using the Mbio Snap-ESI fluorescence waveguide reader. The control well shows no background binding.

Contact Information

Name

Dr Caroline Muphy

Telephone

+353 1 700 8324

E-mail

caroline.s.murphy@dcu.ie

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

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