Anti-Myeloperoxidase (MPO) scFv (B10B)

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

Overview

Antigen Target

Myeloperoxidase (MPO)

Epitope

Not defined

Antibody type/clonality

Recombinant scFv antibody

Clone Number

B10B

Source/Host

Chicken

Species Reactivity

Applications

ELISA, WB

Properties

Form

Liquid

Purity

Specificity

High

Affinity

1.86 X 10-10M

Concentration

1.52 mg/mL

Application working concentration

ELISA (1:1,000-1:10,000)
Western blotting (1:1,000-1:5,000)

Storage Buffer

1 x PBS

Supply & Storage

Storage Conditions

-20oC in 1 x PBS. Avoid repeated freeze and thaw cycles.

Standard Shipping Volumes

200 μL

Shipping Conditions

Additional Information

Reference on Antibody

McDonnell B. (2010) Development of novel antibody-based diagnostics for the early and rapid detection of cardiac markers. PhD thesis, Dublin City University.

Application Information

SDS-PAGE, Western blotting and ELISA analysis of anti-MPO scFv, B10B (Fig.1-2)

Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-MPO scFv.

Figure 1a shows the SDS-PAGE containing purified anti-MPO scFv (~ 28 kDa) and the anti-MPO scFv-specific band on Western blotting was probed with mouse anti-HA IgG. MW: Pre-stained protein ladder; E: elution. The figure 1b shows the schematic diagram of the Western blotting analysis.

Fig. 2 Direct ELISA analysis of purified anti-MPO scFv titre.

Figure 2a shows the titre determined as the scFv concentration required to produce a signal 3 times greater than the background signal. The figure 2b shows the schematic diagram of the ELISA analysis.

Contact Information

Name

Dr Caroline Murphy

Telephone

+353 1 700 8324

Address

School of Biotechnology,
Dublin City University,
Glasnevin,
Dublin 9,
Ireland.

PDF Spec Sheet

Download a pdf version of this antibody spec sheet here.

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