SDS-PAGE, Western blotting and ELISA analysis of anti-MPO scFv, B10B (Fig.1-2)
Fig. 1 SDS-PAGE and Western blotting analysis of purified anti-MPO scFv.
Figure 1a shows the SDS-PAGE containing purified anti-MPO scFv (~ 28 kDa) and the anti-MPO scFv-specific band on Western blotting was probed with mouse anti-HA IgG. MW: Pre-stained protein ladder; E: elution. The figure 1b shows the schematic diagram of the Western blotting analysis.
Fig. 2 Direct ELISA analysis of purified anti-MPO scFv titre.
Figure 2a shows the titre determined as the scFv concentration required to produce a signal 3 times greater than the background signal. The figure 2b shows the schematic diagram of the ELISA analysis.